Automated determination of serum glutamic oxaloacetic transaminase.

A simplified and precise automated method is described for the colorimetric determination of serum glutamic oxaloacetic transaminase. The enzymically generated oxaloacetic acid is dialyzed, coupled with Fast Ponceau L, a diazonium salt, to form a colored solution whose absorbance is measured at 455 m . Dialysis eliminates the need for a serum control determination, thereby halving the analysis time, which is usually 1 hr. per 40 specimens. Comparison of values obtained using this procedure with and without dialysis shows excellent agreement. Comparison with the spectrophotometric procedure shows good correlation. The results of sample interaction experiments are also presented.